Research Associate - Pouladi Lab
Academic
Job CategoryFaculty Non Bargaining
Job TitleResearch Associate - Pouladi Lab
DepartmentPouladi Laboratory | Department of Medical Genetics | Faculty of Medicine (Mahmoud Pouladi)
Posting End DateNovember 30, 2024
Note: Applications will be accepted until 11:59 PM on the Posting End Date.
Job End DateMar 30, 2026
The expected pay range for this position is $5,833.33 - $6,272.40/month
The Department of Medical Genetics at The University of British Columbia (UBC) invites applications for a fixed-term full-time Research Associate.
The UBC Department of Medical Genetics pursues basic and clinical research for the diagnosis, prevention, and treatment of genetic disease. Our goal is to be a world leader in the research, clinical practice, and teaching of Genetic Medicine.The Department is an inspiring and productive community of scholars dedicated to generating and disseminating knowledge and technical expertise in the field of genetics and genomics. We are a respected source of information and advice to society.
While focusing on key areas of genetic medicine, we view diversity of approach and techniques to be an essential strength of our department.
Reporting to Dr. Mahmoud Pouladi, the successful candidate will hold an MD/Ph D in Anatomy, Histology, or Pathology with a focus on neurobiology and 5+ years of postdoctoral experience and have:
At least 10 years' experience in laboratory research involving mouse models.Expertise in histology such as immunofluorescence staining, immunohistochemistry, in situ hybridisation (ISH) labelling, transmission electron microscopy (TEM), Golgi staining, dye tracing for anatomical brain connectivity mapping, whole brain 3D structure staining, clearing and imaging.
Animal husbandry and related skills such as weaning, tailing, mouse behaviour testing, perfusions, tissue microdissection, in vivo delivery in the nervous system, in utero electroporation, and stereotaxic injections.Expertise in molecular biology techniques such as Western blotting, PCR, quantitative PCR, molecular cloning, Co-IP, and Ch IP/CLIP.
Expertise with ex vivo / in vitro cell culture techniques such as organotypic brain slice culture, brain tissue transplantation, primary cell culture, cell line culture, transfections, and lentivirus infection.
Experience working with human pluripotent stem cells and their neural differentiation.
Experience using genome editing using CRISPR/Cas9 in immortalized and human pluripotent stem cell lines.
Responsibilities include: Development and analysis of novel mouse and human pluripotent stem cell models of genetic neurological disorders.
Analysis of brain development defects in mouse models of genetic disorders.Preclinical therapeutic studies in human pluripotent stem cell and mouse disease models.
Performing and/or supervising behavioral and histological analyses of novel mouse models of genetic neurological disorders to elucidate the pathophysiology behind the disease, including managing mouse lines and carrying out relevant procedures such as behavioural experiments, microinjection, tissue collection, various types of sectioning, and staining.
Differentiation of human pluripotent stem cells into neural cell types and organoids.Maintenance, expansion, and analysis of cell line, brain slice, and primary neuron cultures.
Perform CRISPR-Cas9 genome editing, lentivirus infection sh RNA, and transfection to knockout, knockdown, or overexpress to regulate gene expression levels.
Electrophysiological recording, such as multi-electrode array (MEA)
A full job description can be requested at mahmoud.pouladi@ubc.ca.
Please submit your application online at: https://ubc.wd10.myworkdayjobs.com/ubcfacultyjobs. An application package should include a letter of application outlining the applicant's research interests and experience, accompanied by a detailed curriculum vitae and the names of three referees.
Should you have any queries around this position, please contact Human Resources Manager at margarita.reyes@ubc.ca.
The University of British Columbia is a global centre for research and teaching, consistently ranked among the top 20 public universities in the world. Since 1915, UBC's entrepreneurial spirit has embraced innovation and challenged the status quo.UBC encourages its students, staff and faculty to challenge convention, lead discovery and explore new ways of learning. At UBC, bold thinking is given a place to develop into ideas that can change the world.
Our Vision: To Transform Health for Everyone.
Ranked among the world's top medical schools with the fifth-largest MD enrollment in North America, the UBC Faculty of Medicine is a leader in both the science and the practice of medicine. Across British Columbia, more than 12,000 faculty and staff are training the next generation of doctors and health care professionals, making remarkable discoveries, and helping to create the pathways to better health for our communities at home and around the world.
The Faculty - comprised of approximately 2,200 administrative support, technical/research and management and professional staff, as well approximately 650 full-time academic and over 10,000 clinical faculty members - is composed of 19 academic basic science and/or clinical departments, three schools, and 24 research centres and institutes.Together with its University and Health Authority partners, the Faculty delivers innovative programs and conducts research in the areas of health and life sciences. Faculty, staff and trainees are located at university campuses, clinical academic campuses in hospital settings and other regionally based centres across the province.
Equity and diversity are essential to academic excellence. An open and diverse community fosters the inclusion of voices that have been underrepresented or discouraged. We encourage applications from members of groups that have been marginalized on any grounds enumerated under the B.C. Human Rights Code, including sex, sexual orientation, gender identity or expression, racialization, disability, political belief, religion, marital or family status, age, and/or status as a First Nation, Metis, Inuit, or Indigenous person.All qualified candidates are encouraged to apply; however Canadians and permanent residents of Canada will be given priority.